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本文应用IMDM营养液对保存2周的兔角膜14只和正常兔角膜10只进行了24例穿透性角膜移植.移植术后角膜植片透明,术后3、7、14、21天的角膜厚度保存组分别是417±28μm、357±15μm、34917±μ(?)、343±11μm;正常对照组分别是409±34μm、356±10μm、343±17μm、341±20μm,两组比较差异无显著性(P>0.05)。移值术后1个月内皮细胞损失率,保存组为32.9%,正常对照组为29.1%,两组比较差异无显著性(P>0.05).扫描电镜观察显示植片内皮细胞镶嵌结构完整。提示IMDM营养液可保存兔角膜2周.
In this paper, 24 cases of penetrating keratoplasty were performed on 14 corneas of rabbits preserved for 2 weeks and 10 corneas of normal rabbits by using IMDM nutrient solution. The corneal grafts were transparent after transplantation, The thickness of the preservation group were 417 ± 28μm, 357 ± 15μm, 34917 ± μ (?), 343 ± 11μm; the normal control group were 409 ± 34μm, 356 ± 10μm, 343 ± 17μm, 341 ± 20μm, the difference was no Significance (P> 0.05). The endothelial cell loss rate at 1 month after transplantation was 32.9% in the preservation group and 29.1% in the normal control group, with no significant difference between the two groups (P> 0.05). The scanning electron microscope showed that the endothelial cell mosaic structure was intact. Tip IMDM nutrient solution can be stored in rabbit corneas for 2 weeks.