论文部分内容阅读
Bacillus sp.WH4是一株锰氧化能力强的锰氧化细菌,在锰污染的水源净化方面具有较好的应用前景.为提高发酵液中Bacillus sp.WH4的活菌数,应用响应面法优化其发酵培养基.Plackett-Burman设计获得对活菌数有显著影响的4个因素:豆粕粉、KH2PO4、MgSO4、FeSO4;最陡爬坡试验确定中心组合实验的最大响应区域;中心组合设计和响应面分析优化出4个显著因素的最佳浓度.优化后的最适培养基(w/%)组成为:蔗糖2、豆粕粉0.96、K2HPO4 0.8、KH2PO4 0.19、MgSO4.7H2O 0.11、CaCl2 0.02、NaCl 0.5、FeSO4.7H2O 0.02.发酵试验表明,最优培养基的活菌数可达4.2×109 CFU mL-1,比初始发酵培养基提高近3倍.
Bacillus sp.WH4 is a manganese oxidation bacterium with strong manganese oxidation potential, which has a good application prospect in the purification of water contaminated by manganese. To improve the viable Bacillus sp. WSH4 in fermentation broth, the response surface methodology Fermentation medium.Plackett-Burman design to obtain the four factors that significantly affect the viable count: soybean meal, KH2PO4, MgSO4, FeSO4; steepest ascent test to determine the maximum response area of the central composite experiment; central composite design and response surface The optimum concentration of four significant factors was optimized and optimized.The optimum medium (w /%) consisted of sucrose 2, soybean meal 0.96, K2HPO4 0.8, KH2PO4 0.19, MgSO4.7H2O 0.11, CaCl2 0.02, NaCl 0.5 , FeSO4.7H2O 0.02.The fermentation test showed that the viable count of the optimal medium reached 4.2 × 109 CFU mL-1, which was nearly 3 times higher than the initial fermentation medium.