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目的:研究江苏省结直肠癌发病与hMSH2基因IVS12(-6)T>C多态及饮食习惯的相关性。方法:对江苏省金坛、泰兴和淮安地区近2年来新发结直肠癌患者共计108例以及配对180例健康体检者的饮食等生活习惯因素进行调查,提取外周血DNA,应用PCR-DHPLC和DNA序列分析的方法,采用病例对照研究结合现场收集的流行病学资料统计分hMSH2基因IVS12(-6)T>C与结直肠癌发病的关系。结果:hMSH2基因IVS12(-6)T>C在家族性结直肠癌患者中检出率较高,且与散发性结直肠癌病例之间差异有统计学意义(P<0.05),在喜食油炸和腌渍食物组,hMSH2基因IVS12(-6)T>CC突变与正常人群之间也存在明显的统计学差异(P<0.05)。结论:hMSH2基因IVS12(-6)T>C突变,通过遗传性和后天获得性等多重因素影响,使得该突变的携带者有更高的结直肠癌发病风险。
Objective: To study the correlation between the incidence of colorectal cancer and the polymorphism of hSMS2 gene IVS12 (-6) T> C and dietary habits in Jiangsu Province. Methods: A total of 108 cases of colorectal cancer patients with newly diagnosed colorectal cancer in the past two years in Jintan, Taixing and Huaian areas in Jiangsu Province were enrolled in this study, and diet and other lifestyle factors were investigated. The DNA of peripheral blood was extracted and PCR-DHPLC and DNA sequence analysis method, the case control study combined with field collection of epidemiological data statistics hMSH2 gene IVS12 (-6) T> C and colorectal cancer incidence. Results: The detection rate of IVS12 (-6) T> C of hMSH2 gene in familial colorectal cancer patients was higher than that in sporadic colorectal cancer patients (P <0.05) There was also a significant difference (P <0.05) between the IVS12 (-6) T> CC mutation of hMSH2 gene and the normal population in fried and pickled food groups. CONCLUSION: The IVS12 (-6) T> C mutation of hMSH2 gene is affected by multiple factors such as heredity and acquired acquired, which makes the carriers of this mutation have a higher risk of developing colorectal cancer.