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目的:观察生川乌配伍瓜蒌对大鼠长期毒性的影响,探讨该反药对配伍与毒性的关系。方法:SD大鼠80只随机分为生川乌单行(RA)0.3 g·kg-1、生川乌与瓜蒌(1∶1)配伍(RAFT)0.3,0.1 g·kg-1生川乌剂量组、空白对照组,每组20只,雌雄各半,连续ig给药30 d,末次给药24 h后,腹主动脉取血,取脏器计算脏器指数,检测心肌酸激酶(CK),乳酸脱氢酶(LDH)、天门冬氨酸氨基转氨酶(AST),丙氨酸氨基转氨酶(ALT)、肾尿素氮(BUN),血清肌酐(Cr)等生化指标,主要组织器官进行病理学检查。余1/3大鼠停药15 d后检测相应指标。结果:各给药组雌性大鼠肾脏系数高于对照组、胸腺系数低于对照组、红细胞数高于对照组(P<0.05),各给药组雌雄大鼠血清AST,ALT,LDH及BUN水平高于对照组(P<0.05或P<0.01),RA组及RAFT 0.3 g·kg-1组雌性大鼠血清Cr水平高于对照组(P<0.05或P<0.01);RA组雌性大鼠AST,LDH水平高于RAFT 0.1 g·kg-1组(P<0.05或P<0.01);RAFT 0.3 g·kg-1组雌性大鼠AST,ALT,Cr,LDH水平及雄性大鼠AST水平高于RAFT 0.1 g·kg-1组(P<0.05或P<0.01)。主要脏器常规HE染色,RAFT 0.3 g·kg-1及RA组大鼠心、肝、肾均有一定程度损伤。恢复期末,RAFT及RA组雌性大鼠血清AST,ALT水平及雄性大鼠血清ALT水平高于对照组(P<0.05,P<0.01),RAFT及RA组AST,ALT水平差异无统计学意义。结论:在所给剂量下,生川乌对大鼠心、肝、肾有明显毒性,RAFT对大鼠心、肝、肾等器官的毒性与RA组比较无明显差异,RA及RAFT对雌性大鼠毒性强于雄性大鼠。
Objective: To observe the effects of Radix Scutellariae and Radix Paeoniae Alba on the long-term toxicity in rats and the relationship between the anti-drug and compatibility and toxicity. Methods: Eighty SD rats were randomly divided into 3 groups: Radix Astragali (RA) 0.3 g · kg-1, Radix Astragali and Radix Paeoniae Alba (RAFT) 0.3,0.1 g · kg-1 Dose group and blank control group. Each group consisted of 20 males and half males with continuous ig administration for 30 days. After the last administration for 24 hours, blood was taken from abdominal aorta and viscera index was calculated. ), Lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT), renal urea nitrogen (BUN), serum creatinine (Cr) and other biochemical indicators of the main tissues and organs of the disease Physical examination. About one-third of rats were stopped for 15 days and then tested the corresponding indicators. Results: The renal coefficient of female rats in each administration group was higher than that of the control group, the thymus coefficient was lower than that of the control group, and the number of erythrocytes was higher than that of the control group (P <0.05). The serum AST, ALT, LDH and BUN (P <0.05 or P <0.01). Serum Cr levels in female RA rats and RAFT 0.3 g · kg -1 group were significantly higher than those in control group (P <0.05 or P <0.01) AST, LDH in RAFT group were significantly higher than those in RAFT 0.1 g · kg-1 group (P <0.05 or P <0.01); AST, ALT, Cr and LDH levels in female rats with RA of 0.3 g · kg- Higher than RAFT 0.1 g · kg-1 group (P <0.05 or P <0.01). HE staining of major organs, RAFT 0.3 g · kg-1 and RA rats heart, liver and kidney have a certain degree of damage. At the end of recovery period, serum AST and ALT levels in female rats and serum ALT levels in male rats were significantly higher than those in control group (P <0.05, P <0.01). There was no significant difference in AST and ALT between RAFT and RA groups. CONCLUSION: Shengchuanwu is obviously toxic to heart, liver and kidney of rats at the given dose. The toxicity of RAFT to heart, liver and kidney in rats is not significantly different from that in RA group. RA and RAFT have significant effects on female rats Toxicity in rats is stronger than in male rats.