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目的 已证实色素上皮衍生因子 (pigment epithelium- derived factor,PEDF)对中枢神经细胞有抗凋亡作用。本实验评价其对压力诱发的视网膜缺血再灌注的影响。 方法 经前房灌注维持眼压 110mm Hg(1mm Hg=0 .133k Pa) ,45 min,建立大鼠视网膜缺血再灌注模型。随后立即向玻璃体注射 10 μ1(0 .1μg/ μl) PEDF,分别于 2 d和 7d摘除眼球 ,测量塑料包埋切片的平均视网膜内层厚度 (mean thicknessof inner retinal layer,MTIRL)和视网膜节细胞 (retinal ganglion cells,RGC)计数。 结果 PEDF玻璃体注射 7d后治疗组的 MTIRL和 RGC明显高于对照组 [(118.1± 5 .0 )μm对 (94.9± 3.0 )μm,P<0 .0 5 ;(6 .0±1.0 )个 / 10 0μm对 (4.5± 0 .5 )个 / 10 0μm,P<0 .0 5 ]。 结论 玻璃体内注射 PEDF有助于防止视网膜缺血再灌注后神经变性和细胞死亡
Objective It has been demonstrated that pigment epithelium-derived factor (PEDF) has an anti-apoptotic effect on central nervous system cells. This experiment evaluated its effect on stress-induced retinal ischemia-reperfusion. Methods Retinal ischemia-reperfusion model was established by maintaining the intraocular pressure of 110mm Hg (1mm Hg = 0.133k Pa) for 45 minutes via anterior chamber perfusion. Immediately thereafter, 10 μl (0.1 μg / μl) of PEDF was injected into the vitreous and the eyeballs were removed on days 2 and 7, respectively. The mean thickness of the inner retinal layer (MTIRL) and the retinal ganglion cells retinal ganglion cells, RGC) count. Results MTIRL and RGC in the treatment group after PEDF vitreous injection for 7 days were significantly higher than those in the control group [(118.1 ± 5.0) μm vs (94.9 ± 3.0) μm, P <0.05; (6.0 ± 1.0) 10 0 μm pairs (4.5 ± 0.5) cells / 100 μm, P <0.05). Conclusion Intravitreal injection of PEDF helps prevent neurodegeneration and cell death after retinal ischemia-reperfusion