论文部分内容阅读
油脂是植物中广泛存在且参与众多生物学过程的一类物质,具有重要的生物学功能.本研究从拟南芥和水稻油脂代谢基因出发,基于序列相似和功能域相同原则,克隆并注释了1003个玉米油脂代谢相关基因,其中,774个从GenBank中直接注释,229个为新克隆基因.电子表达谱分析发现97个基因在玉米籽粒、根、叶或分生组织中特异高表达.整合已发表籽粒油分相关QTL,在玉米基因组中发现70个控制籽粒油份的QTL富集区(分布于34%的基因组区域),其中59个QTL富集区内存在油脂代谢候选基因147个.在玉米籽粒中特异高表达的13个脂肪酸合成代谢基因都落在QTL富集区.本研究挖掘的油脂代谢相关基因信息,电子表达谱数据及整合的油份QTL结果,为玉米油份QTL的进一步精细定位和克隆、玉米籽粒油份形成的分子生物学基础研究提供了有益信息.
Oil is a kind of substance that exists widely in plants and participates in many biological processes, and has important biological functions.In this study, oil metabolism genes from Arabidopsis and rice originated from oil and cloned and annotated based on the same principle of sequence similarity and function domain 1003 genes related to maize lipid metabolism, of which 774 were annotated directly from GenBank and 229 were new cloned genes.Expression of 97 genes was found to be highly expressed in maize kernels, roots, leaves or meristems Seven QTLs for controlling grain oil were found in the maize genome (distributed in 34% of genomic regions), of which 59 are oil metabolism candidates in 59 QTL-rich regions. All of the 13 fatty acid anabolic genes that are highly expressed in maize kernels all fall in the QTL-rich region.The QTLs for oil metabolism genes, electronic expression data and integrated QTLs for oil metabolism in this study were further studied Fine mapping and cloning, corn seed oil formation molecular biology basic research provides useful information.