论文部分内容阅读
目的：探讨丙型病毒性肝炎（ 丙肝） 患者的细胞免疫水平及其在丙肝发病机理中的作用。方法：用生物素链霉亲和素法对１１２ 例抗 Ｈ Ｃ Ｖ 阳性患者外周血单个核细胞进行 Ｔ 细胞亚群及植物血凝素（ Ｐ Ｈ Ａ） 诱导前后ｍ Ｉ Ｌ２ Ｒ 的检测。结果：急慢性丙肝患者与正常对照相比，外周血 Ｃ Ｄ３ ＋ 、 Ｃ Ｄ４ ＋ 百分率降低， Ｃ Ｄ８ ＋ 百分率增高， Ｃ Ｄ４ ＋ ／ Ｃ Ｄ８ ＋ 比值下降（ Ｐ＜ ０ ．０５ ～ Ｐ＜ ０ ．０１） 。外周血单个核细胞在静息状态和在 Ｐ Ｈ Ａ 诱导状态，其ｍ Ｉ Ｌ２ Ｒ 表达水平与正常对照组相比均低下（ Ｐ＜ ０ ．０１） ，但急慢性丙肝患者中 Ｔ 细胞亚群及ｍ Ｉ Ｌ２ Ｒ 表达水平类似（ Ｐ＞ ０ ．０５） 。结论：丙肝患者体内存在明显的细胞免疫功能紊乱， Ｔ 细胞活化障碍，并与肝病的慢性化有关。
Objective: To investigate the level of cellular immunity in patients with hepatitis C (HCV) and its role in the pathogenesis of hepatitis C. Methods: Using biotin-streptavidin method, 112 cases of anti-H C V positive peripheral blood mononuclear cells were T cell subsets and phytohemagglutinin (PHA) before and after induction of m I L 2 R The test. Results: Compared with the control group, the percentage of C D3 + and C D4 +, the percentage of C D8 + increased and the ratio of C D4 + / C D8 + decreased (P <0.05 ~ P <0.05). 01). Peripheral blood mononuclear cells in the resting state and P H A-induced state, m I L 2 R expression levels compared with the normal control group were lower (P <0.01), but acute and chronic hepatitis C patients with T cells Subgroups and m I L 2 R expression levels were similar (P> 0.05). Conclusion: Hepatitis C patients have obvious cellular immune dysfunction, T cell activation disorders, and with chronic liver disease.