论文部分内容阅读
通过对冬季室内和室外分别生长30d的冬小麦在节律性和非节律性融冻变温过程中抗氧化酶活力和渗透调解物含量变化的分析,揭示其在冬小麦适应日融冻胁迫中的作用。结果表明:生育期不同的室内(均温11℃,拔节期)和室外(均温1℃,分蘖静滞期)冬小麦叶片抗氧化酶和脯氨酸对日光强和温度节律变化的响应趋势是一致的,即随日出而增高,中午气温较高时最高,日落而降低;在非节律性变温处理中,室外冬小麦抗氧化酶活力和脯氨酸含量随气温上升至18℃而增高,气温迅速下降到-2.5℃而降低,经历冻-融-冻胁迫冬小麦生长良好。室内冬小麦抗氧化酶活力随气温降低到-6℃,叶片结冻,迅速下降,气温升高到18℃而增加,经历融-冻-融胁迫后植株死亡;室外冬小麦光合速率(Pn)和比室内的低,而抗氧化酶活力高于室内;冬小麦快速提高抗氧化酶活力和脯氨酸含量,抑制氧自由基积累、维护细胞水分平衡,这在适应冬季节律性融冻胁迫中起重要作用;暖冬中冬小麦较高的Pn和较低的抗氧化酶活力可能是引起冬小麦在“倒春寒”中死亡的生理原因。
The changes of antioxidant enzyme activity and osmolyte content in winter and winter during the 30 days of winter wheat rhythm and non-rhythmic freezing and thawing were analyzed to reveal its role in the freeze-thaw-tolerance of winter wheat. The results showed that the trend of daily sunlight intensity and temperature rhythm changes of antioxidant enzymes and proline in winter wheat leaves with different growth stages (average temperature 11 ℃, jointing stage) and outdoor (average temperature 1 ℃, tillering retardation) Is the same, that is, with the sunrise increased, the highest at noon, the highest at sunset, and the sunset to reduce; non-rhythmic temperature control, the outdoor winter wheat antioxidant enzyme activity and proline content increased with the temperature rose to 18 ℃, The temperature dropped rapidly to -2.5 ℃ and decreased. The winter wheat underwent freeze-thaw-freeze stress to grow well. The activity of antioxidant enzyme in winter wheat decreased with temperature to -6 ℃, the temperature decreased rapidly and the temperature increased to 18 ℃, the plant died after thawing-freezing-thawing. The photosynthetic rate (Pn) and ratio Indoor low, and antioxidant enzyme activity higher than indoor; winter wheat quickly increase antioxidant enzyme activity and proline content, inhibition of oxygen free radical accumulation, maintaining cellular water balance, which adapt to winter rhythmic thaw sexual thawing stress play an important role ; Higher Pn and lower antioxidant enzyme activity of winter wheat in mid-winter wheat may be the physiological reason for the death of winter wheat in “late spring cold”.