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用转化生长因子β_1(transforming growth factor β_1,TGFβ_1)作用于入胃腺癌细胞(SGC-7901),48小时后收集细胞爬片。用抗表皮生长因子受体(epidermal growth factor receptor,EGFR)的单抗和免疫组化SABC法及图像分析,发现TGFβ_1作用的胃癌细胞EGFR表达量(107.943±0.202)低于对照细胞(114.298±0.475),差异显著(P<0.05),研究结果提示,TGFβ_1对胃癌细胞增殖的抑制作用可能与其影响EGFR表达数量有关。
Transforming growth factor β_1 (TGFβ_1) was applied to gastric adenocarcinoma cells (SGC-7901). Cells were collected 48 hours later. Using monoclonal antibody against epidermal growth factor receptor (EGFR) and immunohistochemical SABC method and image analysis, the expression of EGFR in gastric cancer cells treated with TGFβ_1 was (107.943±0.202) lower than that of control cells (114.298±0.475). The difference was significant (P<0.05). The results suggest that the inhibitory effect of TGFβ_1 on gastric cancer cell proliferation may be related to its effect on the expression of EGFR.