为优化黑玉米多糖的超声辅助酶提取工艺,应用小组合试验设计,采用响应面法对提取工艺条件进行优化,确定黑玉米粗多糖超声辅助酶提取工艺的最佳条件:超声作用时间33 min,提取温度59℃,纤维素酶添加量1.23%,中性蛋白酶添加量0.93%,在此条件下多糖得率达46.01%,所建立的数学回归模型能够较准确预测黑玉米多糖的得率。 In order to optimize the ultrasound-assisted enzymatic extraction of black corn polysaccharides, a combination of experimental design and response surface methodology was used to optimize the extraction process conditions. The optimum conditions for ultrasonic-assisted enzyme extraction of black corn polysaccharide were determined: ultrasonic time 33 min, The extraction temperature was 59 ℃, the amount of cellulase added was 1.23%, the amount of neutral protease added was 0.93%. Under this condition, the yield of polysaccharides was 46.01%. The established mathematical regression model can predict the yield of black corn polysaccharides more accurately.