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运用荧光定量检测的方法 ,比较了CTAB法、试剂盒法和SDS法提取大豆加工样品的优劣。通过测定其内源基因Lectin得到的Ct值大小比较基因表达水平的高低 ,通过标准加入法得到标记基因 35S和内源基因LectinCt值的差值ΔCt值 ,结合标准曲线 ,计算出每种混合样品中转基因成分的百分含量 ,进而比较这三种提取方法对PCR反应的抑制程度。总体说来 ,CTAB法提取大豆加工样品得到的核酸最适合PCR反应 ,其次是试剂盒法 ,SDS法稍差
Using fluorescence quantitative detection methods, CTAB method, kit method and SDS method to compare the advantages and disadvantages of soybean processing samples. By comparing the Ct values obtained by the endogenous gene Lectin with the comparative gene expression levels, the difference ΔCt value between the labeled gene 35S and the endogenous gene LectinCt values was obtained by standard addition method, and the standard curve was used to calculate the difference between the Ct values in each mixed sample The percentage of transgenic components, and then compare the degree of inhibition of these three extraction methods on the PCR reaction. Overall, the nucleic acids obtained from CTAB extraction of soybeans processed samples were most suitable for PCR reactions, followed by the kit method and the SDS method with slightly less