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南堡凹陷是我国冀东油田黄骅坳陷中的重要构造之一，已发现了重要的生物气藏。文章依据稳定碳同位素分馏原理，分析了取自南堡凹陷３１口井（深度为１０６６．６～４０１６．４ｍ）第三系地层水中ＨＣＯ的碳同位素组成，识别出水中碳酸根离子的两种主要来源：①甲烷菌活动的产物；②有机质热裂解的产物。研究证实，地层水中溶解的ＨＣＯ的δ１３Ｃ值具有良好的示踪作用，而且可以成为判断地层中是否有甲烷细菌发酵作用存在及生物气是否生成的可靠指标。研究还证实，南堡地区的断层具良好的阻挡作用，有利于油气的聚集与保存，区内生物气生成的最佳深度在１５００～２０００ｍ处。
Nanpu Depression is one of the important structures in the Huanghua Depression of Jidong Oilfield in China. Important biogas has been discovered. Based on the principle of stable carbon isotope fractionation, the carbon isotope composition of HCO in the Tertiary formation water from 31 wells (depth 1066.6-4016.4m) in Nanpu Sag was analyzed, and the two main types of carbonate ions in water were identified Source: ① methanogen activity products; ② pyrolysis of organic matter products. The study confirmed that the δ13C value of dissolved HCO in formation water has a good tracing effect and can be a reliable indicator to determine whether there is methane fermentation in the formation and whether biogas is generated. The study also confirmed that faults in Nanpu area have a good barrier and are conducive to the accumulation and preservation of oil and gas. The optimal depth of biogas generation in the area is between 1500m and 2000m.