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应用地高辛标记的人β神经生长因子ＤＮＡ探针原位杂交组化技术，研究雌性ＩＣＲ小鼠颌下腺的神经生长因子（ＮＧＦ）基因表达。颌下腺经４％多聚甲醛固定，常规冰冻切片，并以同种雄性小鼠颌下腺作阳性对照。实验结果显示，雌性小鼠颌下腺的ＮＧＦｍＲＮＡ分布与雄性小鼠相同，主要存在于纹状管和颗粒曲管上皮细胞中，但杂交信号明显弱于雄性小鼠；在原位杂交反应阳性的纹状管或颗粒曲管上皮细胞之间常可见到几个阴性细胞，阳性管道的密度亦显著低于雄性小鼠。研究表明，小鼠颌下腺ＮＧＦ基因的表达受到雄激素的调节，这种调节作用可能发生于转录水平。ＮＧＦ基因转录少，纹状管或颗粒曲管部分上皮细胞ＮＧＦ基因不开放以及颗粒曲管不发达是雌性小鼠颌下腺ＮＧＦ含量明显低于雄性小鼠的主要原因。
Digoxin-labeled human β-nerve growth factor DNA probe in situ hybridization was used to study the expression of nerve growth factor (NGF) gene in the submandibular gland of female ICR mice. Submandibular gland fixed by 4% paraformaldehyde, conventional frozen sections, and the same kind of male mouse submandibular gland as a positive control. The results showed that the distribution of NGF mRNA in the submandibular gland of female mice was the same as that of male mice, which mainly existed in the striated duct and granular tubule epithelial cells, but the hybridization signal was weaker than the male mice. Several negative cells can often be seen between the tube or granular tubule epithelial cells, and the density of the positive duct is also significantly lower than that of the male mice. Studies have shown that the expression of NGF gene in mouse submandibular gland is regulated by androgen, and this regulation may occur at transcriptional level. NGF gene transcription is not small, striated or granular part of the epithelial cells of the NGF gene is not open and granular underdevelopment is the female mouse submandibular gland NGF content was significantly lower than the male mice.