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通过将抗病的ＣＭＶ－ｃｐ基因和抗虫的Ｂｔ－ｔｏｘｉｎ基因分别加上ＧａＭＶ３５Ｓ启动子和终止子，依次插入到同一植物表达载体ｐＥ３的Ｔ－ＤＮＡ上，获得双抗载体ｐＥ１４。然后载体ｐＥ１４以土壤农杆菌ＧＶ３１１－ＳＥ介导转化烟草，再生植株的胭脂碱检测及ＰＣＲ扩增证明ＣＭＶ－ＣＰ基因和Ｂｔ－ｔｏｘｉｎ基因已随Ｔ－ＤＮＡ同时导入烟草植株中。抗病抗虫实验表明转基因植株具有较强的抗病毒ＣＭＶ的能力和抗烟青虫的能力。
The double anti-vector pE14 was obtained by inserting the resistant CMV-cp gene and the insect-resistant Bt-toxin gene into the T-DNA of the same plant expression vector pE3 by adding GaMV35S promoter and terminator respectively. Then the vector pE14 was transformed into tobacco by Agrobacterium tumefaciens GV311-SE. The nopaline detection and PCR amplification of the regenerated plants demonstrated that CMV-CP gene and Bt-toxin gene have been introduced into tobacco plants simultaneously with T-DNA. Disease-resistant and insect-resistant experiments showed that the transgenic plants have stronger anti-viral CMV ability and resistance to tobacco budworm.