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目的：考察碱性成纤维细胞生长因子（ｂＦＧＦ）对局部脑缺血损伤和抗氧酶活性的影响．方法：大鼠用插丝法阻塞大脑中动脉２４ｈ，梗塞灶用ＴＴＣ染色显示，图象分析测量；神经功能缺损采用０－５级评分．分别分析每只大鼠左右两侧大脑的４种抗氧化酶活性水平．结果：ｂＦＧＦ（缺血５ｍｉｎ后以４５μｇ·ｋｇ－１·ｈ－１给予３ｈ）可降低梗塞灶体积和神经功能缺损评分．局部脑缺血升高过氧化氢酶（ＣＡＴ），超氧化物歧化酶（ＳＯＤ）和谷胱甘肽过氧化物酶（ＧＳＨＰＸ）的活性，但使谷胱甘肽Ｓ转移酶（ＧＳＨＳＴ）的活性水平降低．ｂＦＧＦ可在缺血状态下进一步升高ＣＡＴ的活性，但对ＳＯＤ，ＧＳＨＰＸ和ＧＳＨＳＴ的水平没有影响．结论：ｂＦＧＦ对局部脑缺血损伤具有神经保护作用，此作用可能与ＣＡＴ活性水平的升高有关．
Objective: To investigate the effect of basic fibroblast growth factor (bFGF) on the focal cerebral ischemia injury and the antioxidant enzyme activity. Methods: The middle cerebral artery occlusion was occluded for 24 h in rats by plugging method. TTC staining was performed on the infarct area and image analysis was performed. The neurological deficits were assessed by 0-5 scale. The levels of 4 antioxidant enzymes in the left and right brains of each rat were analyzed. Results: bFGF (given at 45 μg · kg-1 · h-1 for 5 h after 5 min of ischemia) decreased infarct volume and neurological deficit scores. Local cerebral ischemia increased catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) activity, but the glutathione S transferase ( GSH ST) activity level decreased. bFGF can further increase the activity of CAT in the ischemic state, but has no effect on the levels of SOD, GSH-PX and GSH-ST. CONCLUSION: bFGF has neuroprotective effect on focal cerebral ischemia injury, which may be related to the increase of CAT activity.