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用美蓝法、谷海瀛镀银法、石炭酸复红法和Blendon镀银法等4种染色方法对变形杆菌进行染色,参照West提出的评分标准进行打分。结果4种染色方法中,谷海瀛镀银法染色效果最佳,鞭毛粗大、清晰,易于观察,其次为石炭酸复红法和Blendon镀银法,美蓝法染色效果最差。鞭毛染色方法首选谷海瀛镀银法,但是应用媒染剂稳定性差,需要加热处理。
The proteobacteria were stained with four kinds of dyeing methods, such as the American blue method, the Gallo silver method, the complex red bicarbonate method and the Blendon silver plating method, and scored according to the grading standard proposed by West. Results Among the four staining methods, Gu Haiying silver staining method was the best, the flagella were coarse, clear and easy to observe. The second was carbolic acid complex red and Blendon silver plating, the lowest was the methylene blue staining. Flagella staining method preferred Gu Hai Ying silver method, but the application of mordant stability is poor, the need for heat treatment.