论文部分内容阅读
本文应用免疫组化和银染技术对 80例大肠组织中的P53蛋白、核仁组成区嗜银蛋白进行测定。结果大肠癌、腺瘤和正常粘膜的P53蛋白表达阳性率分别为 76 7%、1 6 7%、5 0 %,AgNOR颗粒记数分别为 9 3 0± 3 53、5 40± 1 95、4 3 8± 1 2 8。大肠腺癌的P53蛋白表达阳性率和AgNOR记数明显高于大肠腺瘤及正常粘膜 (P <0 0 1 ) ,大肠腺瘤与正常粘膜比较无明显差异 (P >0 0 5)。P53阳性组AgNOR颗粒记数明显高于P53阴性组 (P <0 0 1 )。结果表明 ,两种方法呈正相关 ,联合起来进行检测 ,对大肠癌的良恶性鉴别诊断、恶性程度判定及预后评估有一定临床应用价值。
In this study, immunohistochemistry and silver staining techniques were used to determine the P53 protein in the 80 large intestine tissues and the nucleolar protein composition of the nucleolus. Results The positive rates of P53 protein expression in colorectal cancer, adenoma, and normal mucosa were 76.7%, 16.7%, and 50%, respectively. The number of AgNOR particles was 9 3 0±3 53, 5 40± 1 95, 4 respectively. 3 8±1 2 8. The positive rate of P53 protein expression and AgNOR count of colorectal adenocarcinoma were significantly higher than those of colorectal adenoma and normal mucosa (P < 0.01). There was no significant difference between colorectal adenoma and normal mucosa (P > 0.05). The number of AgNOR particles in the P53-positive group was significantly higher than that in the P53-negative group (P < 0 01). The results showed that the two methods were positively correlated and combined to perform the test. It has certain clinical application value for the differential diagnosis of benign and malignant colorectal cancer, the judgment of malignant degree and the evaluation of prognosis.