论文部分内容阅读
目的:探讨ROCK抑制剂法舒地尔(FSD)对急性脑梗死大鼠的脑保护作用。方法:将95只SD大鼠随机分为模型组(70只)和正常组(25只)。模型组以线栓法制备急性脑梗死大鼠模型,正常组以假手术处理。选择制模成功的50只大鼠,随机分为治疗组和对照组,每组25只。以上3组大鼠在术后清醒(术后6h及30h)2次给药:治疗组给予FSD腹腔灌注,对照组及正常组予生理盐水腹腔灌注。制模54h后观察各组大鼠的神经功能,检测局部脑血流量(rCBF)、脑梗死体积,并以Griess比色法检测大鼠脑内一氧化氮(NO)含量。结果:制模54h后对照组及治疗组大鼠的神经功能评分较正常组高,局部脑血流较正常组减低,脑梗死体积较正常组增加。对照组脑内一氧化氮含量较正常组减少,治疗组脑内一氧化氮含量与正常组相比差异无显著性。治疗组大鼠的神经功能评分较对照组减低,局部脑血流(rCBF)高于对照组,脑梗死体积较对照组减少,脑内一氧化氮含量较对照组升高。结论:FSD能改善动物模型的神经功能,提高缺血脑组织局部血流量,减少脑梗死体积,具有脑保护作用。以上作用与诱导内皮细胞产生的NO有关。
AIM: To investigate the protective effects of fasudil (ROCK inhibitor) on cerebral protection in acute cerebral infarction rats. Methods: 95 SD rats were randomly divided into model group (70) and normal group (25). Acute cerebral infarction rat model was established by thread occlusion in the model group and sham operation in the normal group. Fifty rats with successful modeling were randomly divided into treatment group and control group, with 25 rats in each group. The above three groups of rats were awake after operation (6h and 30h after operation). The rats in the treatment group were given intraperitoneal injection of FSD, and the control group and the normal group were injected intraperitoneally with saline. After 54 h of modeling, the neurological function, the regional cerebral blood flow (rCBF) and the volume of cerebral infarction in each group were observed. The content of nitric oxide (NO) in rat brain was detected by Griess colorimetry. Results: After 54 h of modeling, the neurological scores of the control group and the treatment group were significantly higher than those of the normal group. The local cerebral blood flow decreased and the volume of cerebral infarction increased compared with the normal group. The content of nitric oxide in the control group decreased compared with that in the normal group, and the content of nitric oxide in the treatment group had no significant difference compared with the normal group. The neurological score of the treatment group was lower than that of the control group, while the local cerebral blood flow (rCBF) was higher than that of the control group. The volume of cerebral infarction was decreased compared with that of the control group. The content of nitric oxide in the brain was higher than that of the control group. Conclusion: FSD can improve the neurological function of animal model, increase local blood flow in ischemic brain tissue, reduce the volume of cerebral infarction, and protect brain. The above role and induced endothelial cell NO-related.