PICHIA相关论文
委内瑞拉博别瓦尔生物化学研究所研究出一种用果皮渣生产饲用蛋白的新工艺。这项工艺是先培育一种被称为Pichia spartine(简称P·......
Microorganism-mediated,hexadecyltrimethylammonium chloride(CTAC)-directed(MCD) method was employed in this work to synth......
The methylotrophic yeast Pichia pastoris is a highly successful system for production of a variety of heterologous prote......
CD163 is an endocytic receptor specifically expressed in monocytes/macrophages and belongs to class B of the scavenger r......
根据毕赤酵母对遗传密码的偏爱性,在不改动氨基酸序列的前提下,用化学法合成抗菌肽麻蝇素A的基因片段,合成片段拼接后,与α因子重......
Smad4是新近发现的一种肿瘤抑制基因。在 5 0 %的胰腺癌和 30 %的结肠癌中 ,此基因发生突变或缺失。利用Pichiapastoris酵母表达系......
Secretory expression and characterization of a recombinant-deleted variant of human hepatocyte growt
AIM: To study the secretory expression of human hepatocyte growth factor (hdHGF) gene in Pichia pastoris. METHODS: The f......
Construction, expression and characterization of human interferon α2b-(G4S)n-thymosin α1 fusion prot
AIM: Interferon α2b (IFNα2b) and thymosin α1 (Tα1) exhibit synergic effects in the treatment of hepatitis B and hepa......
Single-chain variable domain fragment (scFv) 637 is an antigen-specific scFv of myasthenia gravis. In this study, scFv a......
用ＰＣＲ方法从ｐＰＡＩＪ．７中扩增人纤溶酶原激活剂抑制物２型（ＰＡＩ－２）基因，与ｐＰＵＣ１８重组，经限制性内切酶片段分析与核苷酸序列分析，获得全长人ＰＡＩ－２基因．ＰＡＩ－２基因与表达载体ｐＰＩＣ９重组，构......
抑制Ⅳ型胶原酶在肿瘤组织中的过度表达及中和其生物活性是阻止肿瘤新生血管形成和肿瘤转移的手段之一。我们曾报道过抗Ⅳ型胶原酶......
目的 研究人血管内皮生长因子 (hVEGF16 5 )基因在Pichia .pastoris酵母中的表达 ,获得高效表达的具有生物学活性的重组VEGF16 5......
Expression of Human Vascular Endothelial Growth Factor (VEGF_(165)) in Pichia pastoris and Its Biolo
Objective To express human vascular endothelial growth factor (hVEGF-{165}) cDNA in %Pichia pastroris, %purify the expr......
目的 :研究人Flt 1胞外区 2 3loopcDNA在Pichia pastoris酵母中的表达 ,获得高效表达的具有生物学活性的重组Flt 1(2 3)。方法 :......
采用PCR扩增hVEGF165基因 ,经DNA序列分析后 ,插入含AOX1启动子和α分泌信号肽序列的Pichi apastoris酵母表达载体中 ,构建重组质......
Pichia pastoris expression system is similar to that of the mammal cell in modification of expressed protein, including ......
High-yield expression of recombinant SARS coronavirus nudeocapsid protein in methylotrophic yeast Pi
AIM:Nucleocapsid (N) protein plays an important role inreproduction and pathological reaction of severe acuterespirator......
目的对人含前导肽的β-NGF基因序列进行体外扩增、克隆及分析鉴定,并将其插入Pichiapastoris酵母分泌型表达载体pPIC9K中进行表达......
The DNA fragment encoding mature Mycobacterium tuberculosis major secretory protein Ag85B was inserted into the Pichia p......
Evaluation of immunogenicity and reactogenicity of recombinant DNA hepatitis B vaccine produced in I
AIM: (1) To gain information on immune responses to an accelerated schedule of 0,1, and 2 mo in paramedical staff and BD......
Obesity is a major health problem across the world,but there are few ways to effectively treat or manage it in the long ......
Expression and Characterization of the Recombinant Human FLT-3 Ligand Extracellular Domain in Pichia
OBJECTIVE The FLT-3 ligand (fms-like tyrosine kinase receptor-3 ligand, FL) is a recently described growth factor affect......
Objective To investigate the effect of glycosylation at Asn302 of pro-urokinase (pro-UK) on the stability in culture sup......
H-FABP is regarded as a tissue-specific protein existing only in myocardial cells. It is released from the cardiac tissu......
China is one of the twelve prolific biological diversity countries in the world, and nematophagous fungol resources are ......
A laccase gene from Trametes sp. 420 was recombinantly expressed in Pichia pastoris,producing the enzyme rLacD. Six muta......
Assembly and Immunogenicity of Human Papillomavirus Type 16 Major Capsid Protein(HPV16 L1) in Pichia
In this study, a recombinant Pichia pastoris expression system was developed to express HPV16 L1 protein that was driven......
To obtain a sufficient amount of glycoprotein for further studying the structure and function of HIV-1 envelope glycopro......
Secretive Expression of Insect Antifungal Peptide-Encoded Genes in Pichia pastoris and Activity Assa
The antifungal peptides, drosomycin (Drs) and its isoform drosomycin-like C (Drs-lC) from Drosophila melanogaster and Th......
Expression and Characterization of an Active Chimeric Protein of Human Extracellular Superoxide Dism
Mammalian cells express two isoforms of Cu-and Zn-containing superoxide dismutases(SODs),CuZnSOD and extracellular SOD(E......
Expression and Characterization of a Recombinant Truncated Capsid Protein of Hepatitis E Virus in Pi
Hepatitis E is an enterically transmitted viral disease caused by infection with hepatitis E virus(HEV).HEV is a nonenve......
Enterovirus type 71(EV71) causes severe hand-foot-and-mouth disease(HFMD) resulting in hundreds of deaths of children ev......
Objectives: To express and characterize NS1 of Indonesian-specific DENV2 virus in Pichia pastoris(P. pastoris).Methods: ......
The enhancing effect of lanthanum on gene expression of recombinant allophycocyanin (rAPC), a potential antitumor medici......
Antimicrobial peptides(AMPs)are making headlines in science because they demonstrate superior microbicidal characteristi......
During the Chinese New Year holidays in 2008, Professor Zhang Nai-Heng, former chair of the Department of Biochemistry, ......
Polyphenol Oxidase, Peroxidase and PhenylalanineAmmonium Lyase Induced in Postharvest Peach Fruitsby
Rhizopus rot of peach fruits could be significantly suppressed by Pichia membranefaciens. Polyphenol oxidase (PPO), per......
［目的］报道酮基还原酶在 Ｐｉｃｈｉａ ｐａｓｔｏｒｉｓ中的表达，探讨提高酮基还原酶表达量的策略．［方 法］用不同浓度的 Ｇ４１８甲醇、不同的 ｐＨ值、不同的诱导时间来考察对酮......
目的 研究毕赤酵母表达的重组HBsAg(Pichia-rHBsAg)在小鼠中诱导T细胞免疫应答的能力,评价疫苗的免疫原性.方法 以rHBsAg免疫雌性B......
Molecular Characterization and Expression Analysis of S1 self-incompatibility Locus-linked Pollen 3.
Gametophytic self-incompatibility (GSI) is controlled by a highly polymorphic locus called the S-locus, which is an impo......
目前常用的重组蛋白表达系统有大肠杆菌系统、活性杆状病毒系统、酵母表达系统以及哺乳动物细胞表达系统等。近来快速发展起来的巴......
Enhancing ergosterol production in Pichia pastoris GS115 by overexpressing squalene synthase gene fr
The present study was designed to determine the effects of copy number variations(CNVs) of squalene synthase 1(SQS1) gen......
卡利比克毕赤酵母(Pichia caribbica)是一株安全无害且对水果采后病害具有生物防治效果的酵母,但其生防效力与化学杀菌剂相比,仍存......
目的诱导毕赤酵母(Pichia pastoris)分泌表达表达致倦库蚊(Culex quinquefasciatus)防御素(defensin)成熟肽,为进一步研究致倦库蚊......
本文通过对荣华二采区10...
