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用直接法制备染色体、病理切片和放射自显影技术,观察3’-甲基-4,4’-二甲基偶氮苯(3’-Me-DAB,三甲基奶油黄)诱发大鼠肝癌的诱癌过程。发现诱癌过程中鼠肝组织内细胞增生活跃:早期为卵圆细胞和残留的肝细胞;中期为增生结节内的肝细胞及汇管区胆管上皮细胞;后期为肝癌细胞。同时也发生了染色体数目及形态的变化。染色体畸变以染色单体型为主,提示致癌剂作用于S和G_2期细胞。1、2号染色体断裂率高于理论值,这可能与染色体结构重排有关。癌变过程中染色体畸变可能有累积和放大。我们还对肝癌的发生与染色体畸变的关系进行了探讨。
Chromosomes, pathological sections, and autoradiography techniques were performed using direct methods to observe the effects of 3’-methyl-4,4’-dimethylazobenzene (3’-Me-DAB, trimethyl cream yellow) on rat liver cancer The process of carcinogenesis. It was found that during the process of carcinogenesis, cell proliferation in the rat liver tissue was active: early oval cells and residual hepatocytes; in the middle stage, hepatocytes in the hyperplastic nodules and bile duct epithelial cells in the portal area; and later in the hepatoma cells. At the same time, chromosome number and morphology have also changed. Chromosome aberrations were dominated by chromatids, suggesting that carcinogens act on S and G2 cells. The breakage rate of chromosome 1 and 2 is higher than the theoretical value, which may be related to the rearrangement of chromosome structure. Chromosomal aberrations may accumulate and amplify during carcinogenesis. We also discussed the relationship between the occurrence of liver cancer and chromosome aberrations.