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[目的]为明确铝(Al)、铅(Pb)及其交互作用对大麦幼苗生长及抗氧化代谢的影响。[方法]采用营养液培养方法,研究了胁迫处理10 d后不同浓度Al、Pb单独及复合胁迫对大麦幼苗株高、根长等生长指标和功能叶超氧化物歧化酶(SOD)、过氧化物酶(POD)的活性以及丙二醛(MDA)、游离脯氨酸含量的影响。[结果]单一Al、Pb胁迫处理均在0.05水平显著抑制大麦幼苗生长,表现为根长、株高等生长指标均在0.05水平显著低于对照。胁迫处理导致大麦叶片中SOD、POD活性及MDA、游离脯氨酸含量上升,且Al处理下随着胁迫浓度增加的幅度加大。Al、Pb复合处理时,大麦幼苗生长受抑制程度及SOD、POD活性和MDA、游离脯氨酸含量高于相应单一Al处理值。植株生长、SOD、POD活性及MDA含量以200μmol/L Al+150μmol/L Pb处理下变幅最大,但游离脯氨酸含量在50μmol/LAl+150μmol/L Pb复合处理时最高。[结论]Al、Pb复合处理引发更严重的生长抑制和氧化胁迫。
[Objective] The research aimed to clarify the effects of aluminum (Al), lead (Pb) and their interaction on the growth and antioxidant metabolism of barley seedlings. [Method] The nutrient solution culture method was used to study the effects of different concentrations of Al and Pb on the growth of barley seedlings and the growth of root length and the activities of superoxide dismutase (SOD), peroxidation (POD) activity and malondialdehyde (MDA), free proline content. [Result] The single Al and Pb stress treatments significantly inhibited the growth of barley seedlings at the level of 0.05. The root growth and plant height growth indices were significantly lower at 0.05 than those of the control. Stress treatment resulted in SOD and POD activity and MDA and free proline content in barley leaves increased, and under Al treatment increased with increasing stress concentration. Al and Pb treatments inhibited the growth of barley seedlings, the activity of SOD, POD and MDA, free proline content were higher than the corresponding single Al treatment. Plant growth, SOD, POD activities and MDA contents were the highest under the treatments of 200μmol / L Al + 150μmol / L Pb, but the contents of free proline were the highest when treated with 50μmol / LAl + 150μmol / L Pb. [Conclusion] Compound treatment of Al and Pb caused more severe growth inhibition and oxidative stress.