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应用短期体内实验,研究当归、五味子、土茯苓和联苯双酯对黄曲霉毒素B_1(AFB_1)致大鼠肝癌作用的影响。纯系雄性Wistar大鼠,8周龄,经腹腔注入AFB_1(280μg/kg,体重/次,6次/周×2),作肝癌启动剂。2周后,施以促癌程序:给含0.015％2—乙酰氨基芴(2AAF)饲料2周,在第1周末,切除大鼠肝中叶和左外侧叶。10天后,断颈处死大鼠,肝脏取材作r—谷氨酰转肽酶(r—GT)染色。大鼠从注射AFB_1前10天起,至停注AFB_1后3天止,进食含受试物饲料。结果,大鼠肝癌前病变r—GT染色阳性肝细胞灶,在当归组,五味子组和联苯双酯组均比对照组病灶要细,数目较少,差别显著或非常显著。土茯苓组的病灶也稍少于且显著小于对照组。以上表明,这些治疗肝炎有效的药物对AFB_1致肝癌作用有显著抑制效果。提示它们对预防肝癌有实用价值。
Short-term in vivo experiments were conducted to investigate the effects of Angelica sinensis, Schisandra chinensis, Radix Scutellariae, and Bifendate on the effect of aflatoxin B 1 (AFB 1)-induced liver cancer in rats. Pure male Wistar rats, 8 weeks old, were injected intraperitoneally with AFB_1 (280 μg/kg, body weight/time, 6 times/week×2) as a liver cancer triggering agent. Two weeks later, a cancer-promoting procedure was performed: the rats were given 0.015% 2-acetylaminoguanidine (2AAF) for 2 weeks. On the first weekend, the middle and left lateral lobes of the rats were excised. Ten days later, rats were killed by cervical dislocation and the liver was harvested for r-glutamyl transpeptidase (r-GT) staining. The rats were fed with the test substance feed starting 10 days before the AFB_1 injection and 3 days after stopping the AFB_1 injection. RESULTS: Rat hepatoma lesions were stained with r-GT positive hepatic cells. In the Angelica group, Wuweizi group and Bifendate group, the lesions were smaller and the number was smaller than the control group. The difference was significant or very significant. The lesions in the bandits group were also slightly less than and significantly smaller than the control group. The above shows that these effective drugs for treating hepatitis have a significant inhibitory effect on AFB 1 -induced liver cancer. It is suggested that they have practical value in preventing liver cancer.