目的：研究卡介苗细胞壁骨架（ＢＣＧＣＷＳ）加白细胞介素２（ＩＬ２）膀胱灌注对肿瘤组织端粒酶活性的影响，从而探讨该治疗的作用机理。方法：采用端粒重复序列扩增法（ＴＲＡＰ）分别检测２０例膀胱肿瘤用药前后端粒酶活性的表达。结果：经分别测定端粒酶提取原液以及１０倍、１００倍稀释液中端粒酶活性的表达，表明用药后肿瘤组织中端粒酶的活性明显降低（Ｐ＜００１）。结论：ＢＣＧＣＷＳ联合ＩＬ２膀胱灌注能有效地抑制肿瘤细胞端粒酶的活性，提示该治疗的作用机理之一可能是肿瘤端粒酶活性的改变。 Objective: To study the effect of BCGCWS combined with interleukin2 (IL2) intravesical instillation on telomerase activity in tumor tissue and to explore the mechanism of action of this treatment. Methods: Telomerase activity was detected by telomeric repeat amplification (TRAP) in 20 cases of bladder cancer before and after treatment. Results: The telomerase activity and the telomerase activity in the 10-fold and 100-fold dilutions were determined respectively. The results showed that telomerase activity in the tumor tissue was significantly decreased (P <001). Conclusion: BCGCWS combined with IL2 intravesical instillation can effectively inhibit the activity of telomerase in tumor cells, suggesting that one of the mechanisms of action might be the alteration of telomerase activity.