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目的:探讨各型乙型肝炎患者血清sIL-2R、IFN-γ和IL-l变化及其临床意义。方法:收集急性肝炎、慢性肝炎、慢性重型肝炙和活动性肝硬变患者血清标本各16份,16例健康人血清标本作为正常对照组,采用酶联免疫吸附试验(ELISA)检测各型乙型肝炎患者血清中sIL-2R和IFN-γ水平,采用MTT法检测各型乙型肝炎患者血清中IL-1水平,并与正常对照组进行比较。结果:急性肝炎患者血清sIL-2R、IFN-γ和IL-1水平均明显升高(P<0.01);慢性肝炎患者血清sIL-2R水平升高(P<0.05),IFN-γ水平无明显变化,IL-1水平稍降低;慢性重型肝炎患者血清sIL-2R和IFN-γ水平升高(P<0.05 and P<0.01 respectiveIy).但IL-1水平降低;活动性肝硬变患者血清sIL-2R和IFN-γ水平明显升高(P<0.01),但IL-1水平无明显变化。结论:血清sIL-2R、IFN-γ和IL-1参与各型乙型肝炎的发病机制,调整血清中细胞因子含量可能有助于乙型肝炎的治疗。
Objective: To investigate the changes of serum sIL-2R, IFN-γ and IL-1 in patients with various types of hepatitis B and their clinical significance. Methods: Serum samples from 16 patients with acute hepatitis, chronic hepatitis, chronic severe hepatobiliary disease and active cirrhosis were collected from 16 patients and 16 healthy volunteers as normal control group. ELISA was used to detect each type of B Serum levels of sIL-2R and IFN-γ in patients with hepatitis B were measured. The level of IL-1 in sera of patients with various types of hepatitis B was detected by MTT assay and compared with the normal control group. Results: Serum levels of sIL-2R, IFN-γ and IL-1 were significantly increased in patients with acute hepatitis (P <0.01). Serum levels of sIL-2R were significantly increased in patients with chronic hepatitis (P <0.05) (P <0.05 and P <0.01), but the level of IL-1 was decreased. The levels of sIL-2R and IFN-γ in patients with chronic severe hepatitis were significantly lower than those in patients with active cirrhosis -2R and IFN-γ levels were significantly increased (P <0.01), but IL-1 levels did not change significantly. Conclusion: Serum sIL-2R, IFN-γ and IL-1 are involved in the pathogenesis of various types of hepatitis B and adjusting serum cytokines may be helpful for the treatment of hepatitis B.