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目的观察旋毛虫及其虫源性蛋白对盲肠结扎穿孔(CLP)诱导的小鼠脓毒症的影响。方法 80只雄性BALB/c小鼠随机分为假手术组、CLP组、旋毛虫肌幼虫(ML)预感染组、旋毛虫肌幼虫虫体可溶性蛋白(SMP)处理组和排泄分泌蛋白(MES)处理组。ML预感染组于术前28 d经口感染300条旋毛虫肌幼虫,其余各组分别于术后30 min腹腔注射PBS或SMP(25μg/只)或MES(25μg/只)。观察小鼠术后状态和72 h生存率,检测术后12 h小鼠血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、尿素氮(BUN)和肌酐(Cr)水平及TNF-α、IL-6、IL-1β、IL-10、TGF-β水平,观察小鼠肝和肾组织病变。结果与假手术组相比,CLP组72 h生存率降低,血清中ALT、AST、BUN和Cr水平及细胞因子TNF-α、IL-6、IL-1β、IL-10和TGF-β水平均明显升高(P<0.05)。肝中肝索排列紊乱,肝细胞水肿,肾中部分血管球皱缩,肾小管细胞水肿。与CLP组相比,ML预感染组血清中ALT、AST、Cr、TNF-α和IL-1β水平降低,IL-10和TGF-β水平升高(P<0.05);SMP处理组血清中ALT、AST、Cr、TNF-α和IL-1β水平降低,TGF-β水平升高(P<0.05);MES处理组72 h生存率明显升高,血清中ALT、AST、BUN、Cr、TNF-α、IL-6和IL-1β水平明显降低,IL-10和TGF-β水平明显升高(P<0.05),肝和肾组织结构损伤明显减轻。结论旋毛虫及其虫源性蛋白可减少CLP诱导的脓毒症小鼠血清中促炎因子的释放,促进免疫调节因子的释放,其中MES效果更为显著,并能减轻肝和肾结构和功能的损伤。
Objective To investigate the effects of Trichinella spiralis and its insect-derived proteins on cecal ligation and puncture (CLP) -induced sepsis in mice. Methods Eighty male BALB / c mice were randomly divided into three groups: sham operation group, CLP group, predominated group of Trichinella spiralis larvae (ML), SMP group and MES (excretory secretory protein) Processing group. In the ML pre-infection group, 300 Trichinella spiralis larvae were orally infected at 28 days before operation. The other groups were intraperitoneally injected with PBS or SMP (25 μg / mouse) or MES (25 μg / mouse) 30 minutes after the operation. The postoperative state and the survival rate at 72 h were observed. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN) and creatinine Cr levels and the levels of TNF-α, IL-6, IL-1β, IL-10 and TGF-β in the liver and kidney of mice. Results Compared with the sham-operation group, the survival rate at 72 h in CLP group was decreased, the levels of ALT, AST, BUN and Cr in serum and the levels of TNF-α, IL-6, IL-1β, IL-10 and TGF- Was significantly higher (P <0.05). Hepatic liver disorder arranged in the liver, liver cell edema, partial renal glomerular contraction, tubular cell edema. Compared with the CLP group, the levels of ALT, AST, Cr, TNF-α and IL-1βwere decreased and the levels of IL-10 and TGF-β were increased in the ML pre-infected group (P <0.05) The levels of AST, Cr, TNF-α and IL-1β were decreased and the level of TGF-β was increased (P <0.05). The survival rates at 72 h in MES group were significantly higher than those in MES group α, IL-6 and IL-1β levels, IL-10 and TGF-β levels were significantly increased (P <0.05), liver and kidney tissue damage was significantly reduced. Conclusion Trichinella spiralis and its insect-derived proteins can reduce the release of proinflammatory cytokines and promote the release of immunomodulatory factors in CLP-induced sepsis mice. MES is more effective and can reduce the structure and function of liver and kidney Damage.