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目的:观察黄芪对发作期哮喘患者T细胞亚群细胞周期的影响,揭示黄芪在哮喘治疗中的意义。方法:课题以儿童哮喘病人T细胞亚群为研究对象,对CD4+、CD8+T细胞进行分离,采用细胞培养技术,用黄芪对其进行体外处理,使用流式细胞仪测定经黄芪处理和未经黄芪处理的CD4+、CD8+T细胞的细胞周期分布情况。结果:儿童哮喘患者CD4+、CD8+T细胞分别在经黄芪处理和未经黄芪处理的条件下培养,在CD4+T细胞中,黄芪组的S期CD4+T细胞百分比显著低于对照组,有统计学意义(P<0.01);G1期细胞百分比显著高于对照组,有统计学意义(P<0.01)。在CD8+T细胞中,黄芪组的S期CD8+T细胞百分比低于对照组,有统计学差意义(P<0.05);G1期细胞百分比高于对照组,有统计学意义(P<0.05)。结论:黄芪抑制发作期哮喘患者CD4+、CD8+T细胞DNA合成,进而调节细胞周期使之停滞在G1期。黄芪对发作期儿童哮喘患者有一定的治疗意义。
OBJECTIVE: To observe the effect of Astragalus membranaceus on the cell cycle of T lymphocyte subsets in patients with asthma and to reveal the significance of astragalus in the treatment of asthma. Methods: T-lymphocyte subsets of children with asthma were studied in this study. CD4 + and CD8 + T cells were isolated and cultured in vitro with Astragalus membranaceus. Cell viability was measured by flow cytometry Astragalus-treated CD4 +, CD8 + T cell cycle distribution. Results: CD4 + and CD8 + T cells in children with asthma were cultured with and without Astragalus, respectively. In CD4 + T cells, the percentages of CD4 + T cells in S phase in Astragalus were significantly lower than those in control group (P <0.01). The percentage of cells in G1 phase was significantly higher than that in control group (P <0.01). In CD8 + T cells, the percentage of S phase CD8 + T cells in astragalus group was lower than that in control group (P <0.05), and the percentage of cells in G1 phase was higher than that in control group (P <0.05 ). Conclusion: Astragalus can inhibit the DNA synthesis of CD4 + and CD8 + T cells in patients with asthma during the attack period, and then regulate the cell cycle to arrest in G1 phase. Astragalus on the onset of childhood asthma patients have a certain therapeutic value.