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近年来,补体在体外对绦虫成虫和幼虫的致死作用,已引起注意。本文观察了细粒棘球绦虫在补体作用下的形态变化。以羊棘球蚴囊感染狗获得成虫,取得100条置于无菌瓶中,加HBSS液1ml,置38～39℃培养。其中18瓶中加入50%豚鼠血清1ml,于5、10、20、30、40、50分钟和1、2、3、4、5、6、7、8小时,弃HBSS液和血清,以30%戊二醛固定;3瓶中加入经56℃30分钟灭活的豚鼠血清1ml,8小时后以及余下的3瓶,也用上法固定。采用光镜、扫描电镜和透射电镜进行观察。
In recent years, the lethal effect of complement on adult tapeworms and larvae in vitro has attracted attention. This article observed the morphological changes of Echinococcus granulosus under the action of complement. In order to obtain the adult dogs infected with echinococcus granulosus, 100 were placed in a sterile bottle, add HBSS solution 1ml, set 38 ~ 39 ℃ culture. One of 18 bottles added 50% guinea pig serum 1ml, at 5,10,20,30,40,50 minutes and 1,2,3,4,5,6,7,8 hours, discard HBSS solution and serum to 30 % Glutaraldehyde; 3 ml of guinea pig serum inactivated at 56 ° C for 30 minutes, 1 ml after 8 hours, and the remaining 3 bottles, were also fixed by the above method. Using light microscopy, scanning electron microscopy and transmission electron microscopy were observed.