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以胡芦巴种子干粉为原料,无水乙醇为提取溶剂,采用冷浸法、温浸法、索氏法、冷浸-索氏法、超临界CO2萃取法提取的胡芦巴粗提物分别对杂拟谷盗的触杀和驱避活性进行了比较研究,5种方法得到的粗提物对杂拟谷盗均表现出良好的驱避性,但不同方法粗提物的有效驱避剂量差异显著。其中,超临界CO2萃取法粗提物试验所用剂量最小,其平均的驱避率为87.9%,驱避级别为Ⅴ级。5种方法得到的粗提物对杂拟谷盗处理48 h的触杀致死中用量从小到大依次为温浸法、超临界CO2萃取法、冷浸法、冷浸-索氏提取、索氏提取法(LD50=0.450,0.474,0.600,0.670,0.678 mg/cm2)。
Using fenugreek seed powder as raw material and anhydrous ethanol as extraction solvent, crude extract of fenugreek extracted by cold soaking method, warm soaking method, soxhlet method, cold soaking - Soxhlet method and supercritical CO2 extraction The comparison of the contact and repellent activities of the miscellaneous grains was carried out. The crude extracts obtained by the five methods showed good repellent to the miscellaneous grains, but the effective repellent doses of the crude extracts of different methods were different Significant. Among them, the supercritical CO2 extraction crude extract test with the smallest dose, the average repellent rate of 87.9%, level of repellency Ⅴ. The results showed that the amount of the crude extracts obtained from the five methods for contact lethality after 48 h treatment were as follows: warm immersion method, supercritical CO2 extraction, cold soaking, cold soaking - Soxhlet extraction, Soxhlet extraction Method (LD50 = 0.450,0.474,0.600,0.670,0.678 mg / cm2).