论文部分内容阅读
已报导的体外培养恶性疟原虫的方法中,蜡烛缸法虽简单并已广泛应用,但需手工操作,换液频繁,大量生产疟原虫受到限制;半自动化的流灌法或倾斜法其装置及制造成本较高,亦不易推广及生产大量的疟原虫。为此,本文介绍一种可以推广的简单装置,成本低廉的大量生产恶性疟原虫的方法。培养器为4只扁平的22×75×400mm长方形玻璃管,每管的两端各有一只具有硅胶塞的圆柱形窗(直径21mm)和一接种瓶样开口(直径为40×14mm),前窗的玻璃及硅胶管分别向上连接到一具4分支的玻璃集合
Has been reported in vitro methods of culturing Plasmodium falciparum, the candle cylinder method is simple and has been widely used, but the need for manual operation, frequent fluid change, mass production of Plasmodium is limited; semi-automated flow irrigation method or tilt method of its device and The manufacturing cost is high, and it is not easy to promote and produce a large amount of plasmodium. To this end, this article describes a simple and inexpensive device that can be used to produce P. falciparum. The incubators consisted of four flat 22 x 75 x 400 mm rectangular glass tubes each with a cylindrical window (21 mm in diameter) and a vial-like opening (40 x 14 mm in diameter) Window glass and silicone tube were connected up to a 4-branch glass collection