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1.还原糖的检测人教版化学选修5(有机化学基础)第80页:在试管中加入2ml 10%NaOH溶液,滴加5%CuSO4溶液5滴,再加入2ml 10%的葡萄糖溶液,加热.观察并记录实验现象.人教版生物必修1(分子与细胞)第18页:先向试管内注入2ml待测组织样液,再向试管内注入1ml斐林试剂(甲液:质量浓度为0.1g/ml的NaOH溶液,乙液:质量浓度为0.05g/ml的CuSO4溶液,等量混合均匀,现配现用).之后将试管放入盛有50~65℃温水的大烧杯中加热约2min,观察试管中出现的颜色变化.
1. Detection of Reducing Sugars PEP 5 (Basics of Organic Chemistry) Page 80: Add 2 ml of 10% NaOH solution to the test tube, add 5 drops of 5% CuSO4 solution, add 2 ml of 10% glucose solution and heat Observe and record the experiment Phenomenon PEP 1 (Molecules and Cells) Page 18: 2 ml of the tissue sample solution is injected into the test tube and 1 ml of Fehling reagent is injected into the test tube (liquid A: 0.1g / ml NaOH solution, and B solution: CuSO4 solution with a mass concentration of 0.05g / ml, mixed in the same amount, and is ready for use.) Afterwards, the test tube is placed in a large beaker containing warm water at 50 to 65 DEG C About 2min, observe the color change in the tube.