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采用含ＨＣＶ三种抗原（Ｃ_（２２），Ｃ_（３３），Ｃ_（１００））的重组免疫印迹试验（ＲＩＢＡ）检测了７１例抗－ＨＣＶ阳性（ＲＩＡ－２法）的慢性非甲非乙型肝炎患者血清并与ＨＣＶＲＮＡ结果进行了比较。ＲＩＢＡ阳性５６例，可疑８例，对应ＨＣＶＲＮＡ检出率分别为９４．６４％（５３／５６）和３７．５％（３／８），ＲＩＢＡ阴性的７例均未检出ＨＣＶ ＲＮＡ。ＨＣＶＲＮＡ阳性病例中ＲＩＢＡ多为阳性，可疑仅占５．３６％（３／５６），无１例阴性。可见，ＲＩＢＡ阳性即表示ＨＣＶ感染且存在病毒血症，ＲＩＢＡ可作为第二代ＲＩＡ或ＥＬＩＳＡ查抗－ＨＣＶ的确证实验。
A total of 71 anti-HCV-positive (RIA-2) chronic non-methotrexate non-methylprednisolone tablets were detected using a recombinant immunoblot assay (RIBA) with three antigens (C 22, C 33, C 100) Serum from patients with hepatitis and compared with HCVRNA results. 56 cases were positive for RIBA and 8 cases were suspicious. The detection rates of HCV RNA were 94.64% (53/56) and 37.5% (3/8) respectively, and no HCV RNA was detected in 7 cases with negative RIBA. RBCs were mostly positive in HCVRNA-positive cases, accounting for only 5.36% (3/56) of suspicious cases, none of them negative. Can be seen, RIBA positive that means HCV infection and the presence of viremia, RIBA can be used as a second generation RIA or ELISA confirmation test for anti-HCV.