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目的 探讨亚致死预缺血强度及保护性时窗。方法 钳夹沙土鼠的双侧颈总动脉制造脑缺血模型 ,应用尼氏染色观察迟发性神经元坏死。结果 3 5min前脑缺血再灌流 7d前 ,1min的预缺血强度没有保护作用 ;2min预缺血强度 ,间隔 6h脑缺血没有保护作用 ,间隔 1d、3d、5d、7d脑缺血有保护作用 ,间隔 15d脑缺血保护作用消失。结论 2min亚致死预缺血导致脑内复杂的分子生物学变化 ,间隔时窗 1d～ 7d对CA1区锥体细胞有保护作用。
Objective To investigate the sub-lethal ischemic intensity and protective window. Methods The bilateral common carotid arteries of the gerbils were used to establish the model of cerebral ischemia, and the delayed neuronal necrosis was observed by Nissl staining. Results Pre ischemic intensity at 1 min before ischemia and reperfusion for 35 min had no protective effect. Preconditioning intensity at 2 min and no cerebral ischemia at 6 h intervals were protective. Cerebral ischemia at 1 d, 3 d, 5 d and 7 d was protected Role, interval 15d cerebral ischemic protection disappeared. Conclusion Submindiasic ischemic preconditioning at 2 min leads to complicated molecular biology changes in the brain, and the protective effect of pyramidal cells in the CA1 area is observed at the time window of 1d ~ 7d.