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目的:探讨Fas/FasL在大鼠视网膜缺血再灌注损伤中的表达与细胞凋亡的关系及碱性成纤维细胞生长因子(basicfibroblastgrowthfactor,bFGF)的影响。方法:前房加压法制作大鼠视网膜缺血再灌注损伤模型,28只大鼠随机分为正常组和手术组,其中手术组大鼠左眼为缺血再灌注组,右眼为治疗组,手术组又按照再灌注后不同时间段分为1,6,12,24,48,72h组。应用末端脱氧核酸转移酶介导的脱氧三磷酸尿苷缺口末端标记法检测视网膜神经细胞凋亡指数(apoptosisindex,AI),免疫组织化学法检测视网膜组织中Fas,FasL的表达。结果:视网膜神经细胞的凋亡出现于再灌注后6h,并逐渐递增,24h达到高峰,48h开始下降,72h组不明显。Fas,FasL表达改变与凋亡的神经细胞改变基本一致。bFGF治疗组各观察指标表达变化规律与缺血组基本一致,但AI值在12,24,48h组明显低于缺血组(P<0.05);Fas表达在6,12,24h组较缺血组显著下降(P<0.05);FasL表达在12,24,48h组较缺血组明显下降(P<0.05)。结论:Fas/FasL死亡诱导系统参与视网膜缺血再灌注损伤,导致神经节细胞的凋亡;bFGF可抑制Fas、FasL的表达,减少神经节细胞凋亡,对视网膜缺血再灌注损伤有治疗作用。
Objective: To investigate the relationship between the expression of Fas / FasL and retinal ischemia-reperfusion injury and the effect of basic fibroblast growth factor (bFGF) on retinal ischemia-reperfusion injury in rats. Methods: A rat model of retinal ischemia-reperfusion injury was established by anterior chamber pressure. 28 rats were randomly divided into normal group and operation group. The left eye of the operation group was ischemia-reperfusion group and the right eye was treated group The operation group was divided into 1,6,12,24,48,72h group according to different time after reperfusion. The retinal neuronal apoptosis index (AI) was detected by terminal deoxynucleotidyl transferase-mediated deoxynucleoside triphosphate nick end labeling. The expression of Fas and FasL in retinal tissue was detected by immunohistochemistry. Results: Apoptosis of retinal neurons appeared at 6h after reperfusion, and gradually increased at 24h, peaked at 48h, but no obvious at 72h. The changes of Fas and FasL were basically consistent with those of apoptotic neurons. The changes of the expression of bFGF in the observation group were basically the same as those in the ischemia group, but the AI values in the bFGF group were significantly lower than those in the ischemia group at 12, 24 and 48h (P <0.05) (P <0.05). The expression of FasL in the 12, 24 and 48h groups was significantly lower than that in the ischemic group (P <0.05). CONCLUSIONS: Fas / FasL death-inducing system is involved in retinal ischemia-reperfusion injury and leads to apoptosis of ganglion cells. BFGF can inhibit the expression of Fas and FasL, reduce the apoptosis of ganglion cells and have a therapeutic effect on retinal ischemia-reperfusion injury .