纸上层析定量时,对于层析纸的要求是有一定厚度、紧密度、均匀性、亲水性、无金属离子杂质等。作者选用国产无灰滤纸、普通滤纸及专供层析用纸、在相同条件下进行了双向和单向的氨基酸分离,并进行比较。在双向分离中,无灰滤纸有很多优点,使色氨酸单独分离,赖氨酸、精氨酸、天门冬氨 For chromatographic quantification on paper, the requirements for chromatographic paper are certain thickness, tightness, homogeneity, hydrophilicity, no metal ion impurities, and the like. The authors used home-made ash-free filter paper, common filter paper, and special-purpose chromatography paper to conduct bidirectional and unidirectional amino acid separations under the same conditions and compared them. In the two-way separation, ashless filter paper has many advantages, so that tryptophan alone, lysine, arginine, aspartame