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采用超高效液相色谱(UPLC),建立不同产地生/制何首乌二苯乙烯苷(TSG)的含量测定方法和指纹图谱。大孔树脂柱处理各样品,UPLC分析,Waters ACQUITY UPLC@BEH C18色谱柱(2.1 mm×50 mm,1.7μm),乙腈-0.5%甲酸溶液梯度洗脱,流速0.3 mL·min~(-1),波长254 nm,柱温(25±5)℃。结果发现大孔树脂分离后药材二苯乙烯苷含量均在25.0%以上;成功建立不同产地生/制何首乌药材UPLC指纹图谱;标定了17个色谱峰,聚类分析不能完全将生/制首乌分开,而主成分分析可明显地将其分为两类;二苯乙烯苷是两类何首乌自变量投影重要性指标(VIP)差异最大的组分。前处理方法可获得高含量的二苯乙烯苷,测定方法简单,灵敏,可靠,可用于生/制何首乌的快速鉴别,可作为何首乌药材整体质量控制的方法之一。
Using ultra-performance liquid chromatography (UPLC), the method of determination of content and fingerprint of TSG was established. Each column was treated with a macroporous resin column and eluted with a gradient ACQUITY UPLC @ BEH C18 column (2.1 mm × 50 mm, 1.7 μm) and acetonitrile-0.5% formic acid at a flow rate of 0.3 mL · min -1 , Wavelength 254 nm, column temperature (25 ± 5) ℃. The results showed that the content of stilbene glycoside was more than 25.0% after the separation of macroporous resin. UPLC fingerprinting was successfully established for the crude drug of Radix Polygoni multiflori. Seventeen chromatographic peaks were calibrated. While the principal components analysis can be clearly divided into two categories; Stilbene glucoside is the most significant component of the importance of projection (Polygonum Polygonum var. The pretreatment method can obtain high content of stilbene glucoside, the determination method is simple, sensitive and reliable, and can be used for rapid identification of polygonum multiflorum and can be used as one of the methods for overall quality control of polygonum multiflorum.