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本文将津白2纯系小白鼠60只.随机等数分三组:AFB_1组、AFB_1+香烟熏组和对照组。AFB_1溶于DMSO.5μg/每日/每只量拌于饲料投食,熏烟组以30g/日烟丝熏烟,经半年实验,肝H.E常规切片.发现两实验组.肝细胞有不典型增生,程度难确定,电镜下.肝细胞主要为变质及轻度胶原增生。但肝印片置Leitz MPV Ⅲ型测定肝细胞核DNA含量,发现三组均属多峰型,对照组与AFB_+烟熏组主峰相当4C处.AFB_1组则在8C处.两实验组间从多倍体与异倍体数量看,则有显著差别(P<0.05).证实AFB_1+烟熏组具有阻止AFB_1诱致小白鼠肝癌变的作用。
In this paper, Jinbai 2 pure mice 60 were randomly divided into three groups: AFB 1 group, AFB 1 + cigarette smoked group and control group. AFB_1 was dissolved in DMSO, 5μg/day/each in the diet, smoked tobacco was smoked in 30g/day, half a year experiment, hepatic HE routine sectioning, and two experimental groups were found. Hepatocytes had dysplasia , The degree is difficult to determine, electron microscopy. Liver cells are mainly metamorphic and mild collagen hyperplasia. However, liver prints of Leitz MPV type III were used to determine the nuclear DNA content of liver cells and found that the three groups were multimodal, and the control group and the AFB_+ smoke group were equivalent at 4C. AFB_1 group was at 8C. The number of ploids and aneuploids showed a significant difference (P<0.05). It was confirmed that the AFB_1+smoked group had the effect of preventing AFB_1 from inducing hepatocarcinogenesis in mice.