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为探讨姜黄素对大鼠肾毒血清肾炎的防治作用 ,为其在临床治疗肾小球肾炎提供实验依据 ,将雄性SD大鼠随机分成3组 ,正常对照组 :从尾静脉注射0.5ml生理盐水 ,每天1次 ,连用2d ;模型组 :从尾静脉注射兔抗鼠肾毒血清0.5ml/d ,连用2d ;姜黄素组 :于尾静脉注射兔抗鼠肾毒血清0.5ml/d ,连用2d ,同时腹腔注射姜黄素50mg/(kg·d)。3组大鼠分别于第3、7、14、28d处死 ,测定各批次大鼠24h尿蛋白、血生化指标 ,并观察肾脏病理组织学改变。结果显示 ,姜黄素组24h尿蛋白明显低于模型组 (P<0.01) ,血肌酐和胆固醇于4周时比模型组低 (P<0.05) ;半定量分析显示姜黄素组肾小球细胞数明显低于模型组 (P<0.01) ,间质炎细胞浸润程度亦较模型组明显减轻 (P<0.01) ;2周后肾小管间质损伤指数姜黄素组明显好于模型组 (P<0.05)。提示姜黄素能明显减轻肾毒血清肾炎所致蛋白尿 ,同时降低血肌酐及胆固醇 ,抑制小球内细胞增殖 ,减轻小管间质损伤及炎细胞浸润 ,具有肾脏保护作用。
In order to explore the preventive and therapeutic effects of curcumin on nephrotoxic serum in rats and to provide an experimental basis for its clinical treatment of glomerulonephritis, male SD rats were randomly divided into three groups: normal control group: 0.5ml normal saline , Once daily for 2 days. The model group: Rabbit anti-rat kidney serum was injected 0.5ml / d from the tail vein for 2 days. Curcumin group: Rabbit anti-rat renal serum was injected into the caudal vein at 0.5ml / d for 2d , While intraperitoneal injection of curcumin 50mg / (kg · d). Three groups of rats were sacrificed on the 3rd, 7th, 14th and 28th day, respectively. The 24h urine protein and blood biochemistry indexes were determined in each batch of rats, and pathological changes of kidney were observed. The results showed that 24h urinary protein in curcumin group was significantly lower than that in model group (P <0.01), serum creatinine and cholesterol were lower at 4 weeks than those in model group (P <0.05); semi-quantitative analysis showed that the number of glomerular cells (P <0.01), and the infiltration of interstitial inflammatory cells in model group was significantly lower than that in model group (P <0.01). After 2 weeks, tubulointerstitial injury index curcumin group was significantly better than model group (P <0.05) ). Tip curcumin can significantly reduce proteinuria caused by nephrotoxic serum nephritis, while reducing serum creatinine and cholesterol, inhibition of cell proliferation in small balls, reduce tubulointerstitial injury and inflammatory cell infiltration, with renal protective effect.