论文部分内容阅读
本试验采用了栽培稻(Oryza sativa)—双丰一号(粳型)、原丰早(籼型)、四倍体H_(924)(籼粳杂交后代),非洲栽培稻O.glaberrima,野生稻O.alta,O.austraIiensis,O.minuta,O.brachyantha,O.punctata等九个材料,测定种胚蛋白质特性及其免疫反应,结果表明:SDS—PAGE电泳图谱中,八个稻种中有六条分子量相同的同源谱带,其中12—20万D.三条;30—43万D.二条;D.67万一条。不同稻种种胚RGL提取液与双丰一号RGL抗体的免疫反应出现沉淀线位置形状相同;其血凝活力的大小与稻种进化程度可能有关,并且均为N-乙酰葡萄糖胺所抑制。
In this study, Oryza sativa - Shuangfeng No.1 (japonica), Happo (indica), tetraploid H_ (924) (indica / japonica cross), O. glaberrima Rice O.alta, O.austraIiensis, O.minuta, O.brachyantha, O.punctata and other nine materials, determination of embryo protein characteristics and immune response, the results showed that: SDS-PAGE electrophoresis, eight rice species There are six homologous bands of the same molecular weight, of which 12-20 million D. Three; 30-43 thousand D. Two; D.67 million one. The immunophenotypes of RGL extract from different rice embryos and Shuangfeng No.1 RGL antibody appeared the same shape and position of precipitation line. The extent of the hemagglutination activity might be related to the evolution of rice varieties, and all of them were inhibited by N-acetylglucosamine.