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空斑技术是发展病毒定量与遗传研究的必要基本方法。1959年Bonifas和Schlesinger首次报道了人类腺病毒的空斑形成。他们发现当细胞维持在高精氨酸量的培养基中,2型腺病毒在KB单层细胞中形成空斑。此后,有关空斑产生的许多文献表明所有血清型的腺病毒都能产生空斑。然而,人类腺病毒的某些型(包括那些高致癌亚组的腺病毒)的空斑技术仍受到阻碍。由于这些病毒产生缓慢发展的小空斑,所以尽管有人宣称KB和FL细胞可以应用,但实际上仅限于HEK(人胚肾细胞)细胞是能给于精确而又可重复结果的细胞。但是HEK细胞的来源问题使得应用受限。因此,有必要选择
Plaque technology is necessary to develop quantitative and genetic testing of the virus. 1959 Bonifas and Schlesinger for the first time reported the formation of human adenovirus plaque. They found that type 2 adenovirus forms plaques in KB monolayers when the cells remain in high amounts of arginine. Since then, many papers on plaque generation have shown that all serotypes of adenovirus can produce plaques. However, plaque techniques are still hampered by certain types of human adenoviruses, including those with high oncogenic subgroups. Since these viruses produce slowly developing small plaques, in spite of the claim that KB and FL cells can be used, actually HEK (human embryonic kidney cell) cells are cells that give accurate and repeatable results. However, the source of HEK cells makes the application limited. Therefore, it is necessary to choose