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目的:探讨SOST基因在人牙周膜细胞矿化诱导过程中的表达变化,为进一步研究SOST基因在牙周组织中的作用提供理论基础。方法:在体外培养条件下,将矿化诱导液作用于人牙周膜细胞(7、14、21d),检测细胞矿化能力、SOST基因以及成骨标志基因的表达变化。采用SPSS 17.0软件包对数据进行统计学分析。结果:随着诱导时间的增加,人牙周膜细胞的碱性磷酸酶染色及矿化钙结节染色程度增加,成骨标志基因以及SOST基因的表达量在矿化诱导第14天及21天后明显升高,差异均有统计学意义(P<0.05),并具有时间依赖性。结论:牙周膜细胞在mRNA水平能够表达SOST,而且矿化诱导作用对人牙周膜细胞中SOST基因的表达有促进作用。提示SOST参与人牙周膜细胞的成骨分化过程,并对牙周组织改建有重要作用。
OBJECTIVE: To investigate the expression of SOST gene during the process of human periodontal ligament cell mineralization and to provide a theoretical basis for further study on the role of SOST gene in periodontal tissue. Methods: In vitro culture conditions, mineralization induced fluid on human periodontal ligament cells (7,14,21 d), detection of cell mineralization, SOST gene and osteoblast marker gene expression changes. Data were statistically analyzed using SPSS 17.0 software package. Results: With the increase of induction time, the alkaline phosphatase staining and calcium mineralization of human periodontal ligament cells increased. The expressions of osteogenic marker gene and SOST gene were increased on the 14th and 21st day after mineralization induction Significantly increased, the differences were statistically significant (P <0.05), and with time-dependent. CONCLUSIONS: Periodontal ligament cells can express SOST at mRNA level, and mineralization induces SOST gene expression in human periodontal ligament cells. Prompted SOST participants periodontal ligament cells osteogenic differentiation process, and reconstruction of periodontal tissue has an important role.