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对深圳首例疑似人禽流感病人的标本,进行了RT-PCR、Real-timePCR检测及病毒分离培养、血清中和试验、抗原比检测及发病早期不同病程多份标本的病毒载量分析;对分离物进行了HA基因、NA基因及M基因的核酸检测。结果表明:患者气管吸出物的H5N1亚型和A型流感病毒的特异核酸均呈阳性,并通过细胞培养分离到禽流感病毒A/Guangdong/2/06(H5N1)株。气管吸取物病毒载量随着病程延长逐渐减少,而血清中和抗体水平逐渐上升达到1:160之后又缓缓下降。A/Guangdong/2/06株8个片段的核苷酸序列显示,其与2005～2006年中国南部的禽流感分离株高度同源,与越南、泰国、印度尼西亚等分离到的禽流感分离株存在明显的差异。
The samples of the first suspected human bird flu in Shenzhen were analyzed by RT-PCR, Real-time PCR, virus isolation and culture, serum neutralization test, antigen ratio test and the viral load analysis of multiple samples at different stages of the disease. Isolates were subjected to nucleic acid detection of HA gene, NA gene and M gene. The results showed that the specific nucleic acids of H5N1 subtype and type A influenza virus were positive in tracheal aspirate and the avian influenza virus A / Guangdong / 2/06 (H5N1) strain was isolated by cell culture. Tracheal aspirate viral load decreased gradually with the course of the disease, while serum neutralizing antibody levels gradually increased to 1: 160 and then slowly decreased. The nucleotide sequence of the 8 fragments of A / Guangdong / 2/06 strain showed high homology with the avian influenza isolates in southern China from 2005 to 2006, and isolates of avian influenza isolates from Vietnam, Thailand and Indonesia Significant differences.