In vitro screening for antitumor potential of four cold-adapted entomogenous fungi

来源 :2015年中国微生物学会学术年会 | 被引量 : 0次 | 上传用户:aihuibulai
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Entomogenous fungus,a promising source for the discovery of new antitumor agents,has received considerable attention from around the world.In the present study,Four cold-adapted entogenous fungi such as Akanthomyces sp.(A01),Gibellula sp.(G01),Hirsutella spp.(H01 and H02) were incubated at 20 ℃ on a rotary shaker at 175 rpm for 7 days.Their fermentation broth and mycelium were extracted by 75 %ethanol,respectively.Based on the theory of liquid-liquid extraction,the warm aqueous suspensions of ethanol extracts were successively extracted with petroleum ether,chloroform,ethyl acetate,and water saturated butanol,respectively.
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