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采用多种处理组合、处理方法以及多种生物学指标,探讨了γ射线与EMS复合处理小麦的诱变效应。试验结果表明:γ射线与EMS复合处理的M_1代损伤随处理剂量的增加而增大,呈累加效应或协同效应。M_1代植株的生物学损伤与幼苗高度及幼苗根尖微核细胞密切相关。γ射线与EMS复合处理比单一处理提高了M_2代诱变效应。适宜的剂量组合可增加M_2代形态突变频率,扩大突变谱,并可增加某些数量性状的变异幅度。其中5.16C/kg的γ射线+0.31%EMS是比较理想的剂量。在复合处理中,可以M_1代幼苗根尖单微核细胞率估测M_2代的诱变效应。
A variety of treatment combinations, treatment methods and a variety of biological indicators to explore the γ-ray and EMS composite wheat mutagenic effects. The experimental results showed that the M_1 generation injury induced by γ-ray and EMS increased with the increase of treatment dose, which showed additive or synergistic effects. The biological damage of M_1 plants was closely related to seedling height and seedling root tip micronucleus. Compared with single treatment, γ ray and EMS complex treatment increased the M_2 generation mutagenesis effect. Appropriate dose combination can increase the M_2 generation mutation frequency, expand the mutation spectrum, and increase the variation range of some quantitative traits. Which 5.16C / kg of gamma rays + 0.31% EMS is the ideal dose. In the composite treatment, M_1 generation of seedling apical monocyte rate can be used to estimate M_2 generation mutagenic effect.