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为寻找海参养殖的新型饲料源,对紫花苜蓿替代海藻的可行性进行初步研究。以30%、60%和100%的替代比例添加于饲料中,以直接使用和发酵处理2种方式饲喂海参30 d,以自配的常规海藻型饲料作为对照。投喂结束后,称量体质量,测肠道消化酶和体腔液免疫酶活力。结果表明:试验Ⅰ组和试验Ⅱ组蛋白酶活力均高于对照组,试验Ⅱ组最高,为(178.86±1.42)U/gprot,且差异显著(P<0.05);试验Ⅱ组淀粉酶活力显著高于对照组(P<0.05),为(266.33±1.55)U/gprot;试验Ⅴ组纤维素酶活力显著高于对照组(P<0.05),为(62.48±0.86)U/gprot;试验Ⅳ组脂肪酶活力显著高于对照组(P<0.05),为(5.21±0.45)U/gprot。试验Ⅰ组和试验Ⅱ组的特殊生长率(SGR)均高于对照组(P<0.05),以试验Ⅱ组最高,为(0.55±0.0017)%/d,试验Ⅴ组最低,为(0.24±0.0014)%/d。酸性磷酸酶(ACP)和碱性磷酸酶(AKP)在试验Ⅰ~Ⅳ组的活力持平或略高于对照组;试验Ⅱ组的超氧化物歧化酶(SOD)活性明显高于对照组(P<0.05),为(82.45±0.46)U/mgprot;试验Ⅰ组和试验Ⅱ组的一氧化氮合酶(NOS)活性均显著高于对照组(P<0.05),分别为(5.06±0.38)和(5.28±0.32)U/mgprot。各酶活性在试验Ⅵ组达到最低。结论,在海参饲料中加入紫花苜蓿30%是可行的。
In order to find a new source of feed for sea cucumber cultivation, the feasibility of replacing algae with alfalfa was studied. The sea cucumber was fed for 30 days with 30%, 60% and 100% substitution ratio respectively. The sea cucumber was fed for 30 days by direct use and fermentation treatment. After the end of feeding, weighed the body weight, measured intestinal digestive enzymes and body cavity fluid immune enzyme activity. The results showed that the activities of protease in group Ⅰ and group Ⅱ were higher than those in control group. The highest in group Ⅱ was (178.86 ± 1.42) U / gprot, with significant difference (P <0.05). The activity of amylase in group Ⅱ was significantly higher (266.33 ± 1.55) U / gprot in the control group (P <0.05). The activity of cellulase in group V was significantly higher than that of the control group (62.48 ± 0.86) U / gprot; The lipase activity was significantly higher than that of the control group (P <0.05), which was (5.21 ± 0.45) U / gprot. The SGR of group Ⅰ and group Ⅱ were significantly higher than that of control group (P <0.05). The highest SGR of group Ⅱ was (0.55 ± 0.0017)% / d and the lowest was group Ⅴ (0.24 ± 0.0014)% / d. The activities of ACP and AKP were the same or slightly higher than that of the control group in the experimental group Ⅰ ~ Ⅳ. The activity of superoxide dismutase (SOD) in the experimental group Ⅱ was significantly higher than that of the control group (P (P <0.05), which was (82.45 ± 0.46) U / mgprot. The activity of nitric oxide synthase (NOS) in group Ⅰ and group Ⅱ were significantly higher than that in control group (5.06 ± 0.38) And (5.28 ± 0.32) U / mgprot. Each enzyme activity reached the lowest in group VI. In conclusion, it is feasible to add alfalfa 30% to sea cucumber feed.