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探讨铁对 ＨＬ－６０细胞增生及化疗药物诱导 ＨＬ－６０细胞凋亡的影响，采用ＨＬ－６０细胞与不同浓度的三氯化铁、三氯化铁加阿糖胞苷、三氯化铁加足叶乙甙、阿糖胞苷、足叶乙甙共同体外培养６ｈ、１２ｈ、２４ｈ、４８ｈ，用细胞生长、活力测定、形态学观察、流式细胞学检测分析和ＤＮＡ电泳，免疫组化方法检测ｂｃｌ－２基因等细胞凋亡指标观察三氯化铁的作用。结果显示，１００μｍｏｌ／Ｌ三氯化铁促ＨＬ－６０细胞增生最明显，凋亡率最低，ｂｃｌ－２阳性表达率最高，１００μｍｏｌ／Ｌ三氯化铁分别加用阿糖胞苷、足叶乙甙与 ＨＬ－６０细胞共同培养时凋亡率比单用阿糖胞苷、足叶乙甙低（Ｐ＜０．０５）。可以认为一定量的铁有促进 ＨＬ－６０细胞增生、抑制 ＨＬ－６０细胞凋亡的作用，对于白血病患者，不适当的铁剂补充可能带来不利的影响。
To investigate the effect of iron on the proliferation of HL-60 cells and the apoptosis of HL-60 cells induced by chemotherapeutic drugs. HL-60 cells were used together with different concentrations of ferric chloride, ferric chloride plus cytarabine, and ferric chloride. Etoposide, Ara-C, and Etoposide were co-cultured in vitro for 6 h, 12 h, 24 h, and 48 h, and assayed by cell growth, viability, morphological observation, flow cytometric analysis and DNA electrophoresis, and immunohistochemistry method. Detection of bcl-2 gene and other apoptosis indicators to observe the role of ferric chloride. The results showed that 100μmol/L ferric chloride promoted the proliferation of HL-60 cells, the apoptosis rate was the lowest, and the positive rate of bcl-2 expression was the highest. 100μmol/L ferric chloride was added with cytarabine and acetylcholine B respectively. The co-cultured with HL-60 cells was lower than that of cytarabine alone and etoposide (P<0.05). It can be considered that a certain amount of iron promotes the proliferation of HL-60 cells and inhibits the apoptosis of HL-60 cells. In patients with leukemia, inappropriate iron supplementation may cause adverse effects.