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本研究取自同一养殖海域不同浮筏养殖笼中健康和患脓包病虾夷扇贝(Patinopecten yessoensis)样品,健康虾夷扇贝样品标记为CK,患病样品分别标记为S1、S2和S3,使用Mi Seq高通量测序技术比较不同生存状态的虾夷扇贝的微生物组成与差异。结果表明:4个样品中菌群多样性表现为S3>CK>S1>S2;4个样品中的细菌可归为24个门,主要门类有变形菌门、拟杆菌门、浮霉菌门、梭杆菌门、厚壁菌门、酸杆菌门、放线菌门、硝化螺旋菌门、绿菌门和绿弯菌门;在健康虾夷扇贝样品中,变形菌门占绝对优势,占整个菌群的95.9%,患病虾夷扇贝样品中,S1和S3中优势类群均为变形菌门,分别占整个菌群的80.4%和86.8%,S2中优势菌群为拟杆菌门,占56.9%。
In this study, healthy and pustular Patinopecten yessoensis samples were collected from cages of different raft farms in the same aquaculture area. Samples of healthy Japanese shrimp were screened as CK, and diseased samples were labeled as S1, S2 and S3 respectively. Mi Seq High-Throughput Sequencing Technologies to Compare Microbial Compositions and Differences in Different Living States of Eriocheir japonica. The results showed that the bacterial diversity in the four samples was S3> CK> S1> S2. The bacteria in the four samples could be classified into 24 types. The main categories were Proteobacteria, Bacteroidetes, Bacteroidetes, Firmicutes, Acidobacteria, Actinobacteria, Nitrosospirillum, Chlorophyta and Chlorophyta; in the samples of healthy Japanese shrimp, the Proteobacteria accounted for the absolute advantage, accounting for the entire flora 95.9% of the samples. In the diseased Xiyi scallop samples, the dominant groups in S1 and S3 were all Proteobacteria, accounting for 80.4% and 86.8% of the total, respectively. The dominant bacteria in S2 were Bacteroidetes, accounting for 56.9%.