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无论在温室还是在大田条件下,菌根(AM)真菌均能够侵染冬小麦,但其对冬小麦锌(Zn)吸收的效应及机制尚不明确。本研究将摩西管柄囊霉Funneliformis mosseae和根内根孢囊霉Rhizophagus intraradices分别接种于冬小麦Triticum aestivum(品种:小偃22)根围,10周后分别利用菌根化幼苗进行短期(0–90min)Zn吸收动力学试验和长期(0–210min)Zn吸收积累试验,并研究AM真菌侵染对冬小麦根系形态特征的影响。结果表明,AM真菌通过增加根系长度和根尖数量来扩大冬小麦根系Zn吸收的有效面积、Zn最大吸收速度V_(max)和Zn~(2+)流入根系的速度α,进而促进冬小麦根系对Zn的吸收。接种摩西管柄囊霉降低了K_m值而接种根内根孢囊霉增加了K_m值,这可能与不同AM真菌对冬小麦根系形态影响及对Zn转运蛋白基因表达的影响存在差异有关。
Mycorrhizal (AM) fungi are able to infect winter wheat both in greenhouses and in field conditions, but its effect on zinc uptake in winter wheat is unclear. In this study, Funneliformis mosseae and Rhizophagus intraradices of Rhizophagus intraradices were inoculated into the rhizosphere of Triticum aestivum (variety: Xiaoyan 22) respectively. After 10 weeks, the mycorrhizal seedlings were used for short-term (0-90 min ) Zn absorption kinetics test and long-term (0-210min) Zn absorption accumulation test, and study the AM fungal infection on the root morphology of winter wheat. The results showed that the AM fungi increased the effective area of root Zn absorption, V max and the rate of Zn 2+ influx into root system of winter wheat by increasing root length and root tip number, Absorption. Inoculation with M. gossypii reduced K_m and inoculation with S. ginnala increased K_m, which may be related to the effect of different AM fungi on the root morphology of winter wheat and Zn transporter gene expression.