方法自人体皮肤霉菌病分离出白色念珠菌,置沙包氏葡萄糖琼脂培养基25℃下培养72小时,由血细胞计数器调制成10~6/ml 菌液。实验动物系用体重约1.5kg,健康无眼病的小白兔10只20眼。术前各眼每日结膜下注射0.4%氟美松磷酸钠0.2ml,连用7天,而于术前第5天角膜实质内注入10μl 含约10~4子芽孢的菌液,作为实验模型。 Methods Candida albicans was isolated from mycotic dermatosis of human skin and cultured in Sabouraud dextrose agar medium at 25 ° C for 72 hours. The cell suspension was prepared from 10 ~ 6 / ml bacterial cells by a hemocytometer. Experimental animals with a body weight of about 1.5kg, 10 healthy eyes without eye rabbit 20. Before surgery, each eye was injected subcutaneously with 0.4% sodium dexamethasone 0.2ml daily for 7 days. In the 5th day before operation, 10μl of bacterial liquid containing 10 ~ 4 spores were injected into the cornea of ​​the eye as the experimental model.