论文部分内容阅读
用扫描分光光度计对酶所催化的反应进行化学计量学研究。在反应开始后的不同时间，在２００～４００ｎｍ波长范围内扫描，观察到不同的吸收波谱，直到吸收波谱不再改变为止，由此得到间羟苯甲酸４羟化酶催化间羟苯甲酸转变成３，４二羟苯甲酸随时间变化的吸收波谱。用摩尔消光系数计算每种成分的浓度，在２次独立的实验中，底物与产物的摩尔比值分别为１：０．９９８和１：１．０８５，提示来源于Ｃｏ．ｔｅｓｔｏｓｔｅｒｏｎｉＫＨ１２２３Ｓ菌株的ＭＯＢ４ＨＯａｓｅ与文献报道的来源于Ａｓｐｅｒｇｉｌｕｓｎｉｇｅｒ菌株的ＭＯＢ４ＨＯａｓｅ所催化的反应是相同的。
The reaction catalyzed by the enzyme was stoichiometrically investigated with a scanning spectrophotometer. At different times after the start of the reaction, scanning was performed in the wavelength range of 200-400 nm, and different absorption spectra were observed until the absorption spectrum did not change any more, thereby the m-hydroxybenzoate 4-hydroxylase catalyzed the conversion of m-hydroxybenzoate As 3,4-dihydroxybenzoic acid absorption spectra over time. The concentration of each component was calculated using the molar extinction coefficient. The molar ratio of substrate to product was 1: 0.998 and 1: 1.085, respectively, in 2 independent experiments, suggesting that the source was Co The testosteroni KH122 3S MOB4 HOase strains reported in the literature from the Aspergilus niger MOB4 HOase catalyzed reaction is the same.