论文部分内容阅读
目的 探讨肾移植患者外周血中细胞因子基因多态性与急性排斥反应的关系。方法 应用聚合酶链反应测定 89例肾移植患者外周血中肿瘤坏死因子 (TNF α)、转化生长因子 (TGF β)、白细胞介素 6(IL 6)及干扰素 (IFN γ)的基因型 ,并结合HLA配型情况 ,比较各基因型别的急性排斥反应发生率。结果 在HLA DR错配的情况下 ,TNF α和IFN γ等位基因为高分泌型者 ,其术后急性排斥反应发生率较低分泌型者高 (P <0 .0 5) ;未能显示TGF β及IL 6基因表达与急性排斥反应的相关性。结论 术前检测肾移植患者细胞因子基因型 ,有助于发现可能发生急性排斥反应的高危人群 ,据此可制定合理的个体化免疫抑制治疗方案
Objective To investigate the relationship between cytokine gene polymorphisms in peripheral blood and acute rejection in renal transplant recipients. Methods The genotypes of tumor necrosis factor (TNF), transforming growth factor (TGFβ), interleukin 6 (IL 6) and interferon (IFN γ) in peripheral blood of 89 renal transplant recipients were determined by polymerase chain reaction Combined with HLA matching situation, compare the genotypes of acute rejection rate. Results In the case of HLA DR mismatch, TNFα and IFNγ alleles were highly secreted, with a higher incidence of postoperative acute rejection (P <0.05) Correlation between TGF β and IL 6 gene expression and acute rejection. Conclusions Preoperative detection of cytokine genotypes in renal transplant patients may help to identify high-risk groups at risk of developing acute rejection and thus develop a rational individualized immunosuppressive regimen